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. 2024 Oct 12;15:8830. doi: 10.1038/s41467-024-53173-w

Fig. 2. LPTCs are required for binocular control of walking.

Fig. 2

a Maximum z-projection of Kir2.1::EGFP expression by the VT058487-GAL4 line used to silence HS and VS cells. The flies also carried a tsh-GAL80 transgene to remove GAL4 expression in the ventral nerve cord. b Angular speed of HS & VS-silenced flies in response to FtB and BtF rotation (mean ± SEM). c Comparison of the predicted and the actual mean angular speed in response to full-field rotation for HS & VS-silenced flies (mean ± SEM). Shaded region shows the difference between the predicted and actual response. d Same as in (b) but for UAS-Kir2.1 control flies. e Same as in (c) but for UAS-Kir2.1 control flies. f Time series of mean prediction error. g Mean prediction error across the trial period per fly. Bars: mean ± SEM. h Mean of smooth and saccadic angular speed across a trial. Empty and filled markers show the mean per fly and mean ± SEM for each genotype, respectively. i Cumulative angular displacement for syn-saccadic and anti-saccadic turns in one trial. Empty and filled markers show the mean per fly and mean ± SEM for each genotype, respectively. j Mean of smooth (top), syn-saccadic (middle) and anti-saccadic (bottom) cumulative angular displacement per trial. Bars: mean ± SEM. g, j Two-sided Mann–Whitney U-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. No asterisk: not significant. Number of flies: HS,VS>Kir2.1,tsh-Gal80 = 21, emptyGal4>Kir2.1,tsh-Gal80 = 11. Exact p-values for each experiment are listed in Supplementary Data 1. Source data are provided as a Source Data file. In all panels, black = emptyGal4>Kir2.1, tsh-Gal80 and orange = HS,VS>Kir2.1, tsh-Gal80.