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. 2024 Sep 14;8(11):ziae120. doi: 10.1093/jbmrpl/ziae120

Figure 7.

Figure 7

Th9 and Th17 cells are significantly elevated in post-menopausal osteoporotic patients: (A-B) ELISA data representing the levels of bone biochemical markers viz. CTX-1 and osteocalcin, respectively. (C) tSNE plot for the PBMCs showing expression of IL-9 cytokines gated on CD3+CD4+ Th cells. (D) Zebra plot representing the percentage of Th9 cells. (E) Plot representing the percentage of Th9 cells in control and PMO patients. (F) MFI of IL-9 in CD4+ T cells in HC and PMO patients. (G) Level of IL-9 in sera of HC and PMO patients. (H) Contour plot representing the percentage of Th17 cells in control and PMO patients. (I) Plot represents the percentage of Th17 cells. (J) MFI of IL-17 in CD4+ T cells in HC and PMO patients. (K) Level of IL-17 in sera of HC and PMO patients. (L) Osteoclast differentiation was induced in human PBMCs with M-CSF (25 ng/mL) and RANKL (100 ng/mL) with or without IL-9 cytokine at various concentrations for 14 d. Giant multinucleated cells were stained with TRAP and cells with ≥ 3 nuclei were considered mature osteoclasts. (M) Photomicrographs at 20× magnifications were taken. (N) Number of TRAP positive cells with more than 3 nuclei. Data are expressed as mean ± SEM. Data were analyzed by unpaired Student t test and analyzed by one-way ANOVA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001) compared with the indicated group.