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. 2024 Sep 26;10(19):e38418. doi: 10.1016/j.heliyon.2024.e38418

Fig. 3.

Fig. 3

Histological analysis of various spheroids using DFP (A) Organization of cell spheroids by HaCaT cells on Day 1. Asterisks indicate DFP. (B) Organization of cell spheroids by HaCaT cells on Day 3. Asterisks indicate DFP. (C) Organization of cell spheroids by HaCaT cells on Day 7. Asterisks indicate DFP. (D) Image showing HaCaT cell spheroids stained for the cell proliferation marker Ki67. (E) Image showing HaCaT cell spheroids stained for the cell proliferation marker phospho-Histone H3 (PHH3). Arrows indicate PHH3-positive cells. (F) Image showing HaCaT cell spheroids stained for the cytokeratin marker CK AE1/AE3. (G) Organization of cell spheroids by NIH/3T3 cells on Day 3. Asterisks indicate DFP. (H) Image showing NIH/3T3 cell spheroids stained for the cell proliferation marker PCNA. (I) Image showing NIH/3T3 cell spheroids stained for the myofibroblast marker αSMA. The arrowhead indicates a myofibroblast. (J) Organization of cell spheroids by MS-1 cells on Day 1. Asterisks indicate DFP. (K) Organization of cell spheroids by HMY-1 cells on Day 3. Asterisks indicate DFP. (L) Organization of cell spheroids by CAF cells on Day 7. Asterisk indicates DFP. (M) Organization of cell spheroids by HaCaT cells and NIH/3T3 cells on Day 3. Asterisk indicates DFP. (N) Image showing spheroids of HaCaT cells and NIH/3T3 cells stained for the cytokeratin marker CK AE1/AE3. Arrows indicate CK AE1/AE3 positive HaCaT cells. (O) Image showing spheroids of HaCaT cells and NIH/3T3 cells stained for the intermediate filament marker vimentin. Arrows indicate vimentin positive NIH3T3 cells.