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Western blotting analysis of cell spheroids MAPK family protein, ERK1/2, p-ERK1/2, p38 and p-p38 expression levels in spheroid by HaCaT cell (n = 4) or NIH/3T3 (n = 4) cells were evaluated by western blotting. Relative expression is depicted as the ratio of target protein expression to α/β-tubulin expression. PD, plastic dish; DFP, dried fish powder.
