FIG. 6.

Inhibition of ERK activation does not affect reovirus-induced apoptosis. (A) Reovirus-induced apoptosis (MOI, 100) was measured in the presence of a chemical inhibitor of ERK activation (PD 98059). L929 cells were pretreated with inhibitor (10 μM) for 2 h prior to infection with reovirus and were maintained in medium in the presence of inhibitor for 48 h following reovirus infection. They were then harvested and assayed for apoptosis. The graph shows percent apoptosis. Error bars represent standard errors of the mean. (B) Activation-phosphorylation of ERK following reovirus (T3D) infection (MOI, 100) in the presence of PD 98059 (10 μM) as determined by Western blot analysis using antibodies directed against phospho-ERK.