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. 2024 May 19;57(10):e13657. doi: 10.1111/cpr.13657

FIGURE 3.

FIGURE 3

Activating transcription factor 3 (ATF3)‐induced ferroptosis in senescent osteocytes is not primarily mediated by solute carrier family 7a member 11 (SLC7A11). (A) Western blot analysis of the levels of ATF3, SLC7A11 and glutathione peroxidase 4 (GPX4) in MLOY4 cells at indicated treatment, with quantitative data at right (n = 3). (B and C) Total iron and ferrous iron levels in MLOY4 cells corresponding to the samples in A were quantitatively determined using iron assay kits (n = 3). (D) Representative immunofluorescence of lipid peroxidation (LPO) levels in MLOY4 cells at indicated treatment by using LPO Fluorometric assay kits, with quantitative data on the right (n = 3). (E) Representative images of the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay in MLOY4 cells at indicated treatment, with quantitative data on the right (n = 3). (F) Western blot analysis of the levels of SLC7A11 and GPX4 in MLOY4 cells at indicated treatment, with quantitative data on the right (n = 3). (G and H) Total iron and ferrous iron levels in MLOY4 cells corresponding to the samples in F were quantitatively determined using iron assay kits (n = 3). (I) Representative immunofluorescence of LPO levels in MLOY4 cells at indicated treatment by using LPO Fluorometric assay kits, with quantitative data on the right (n = 3). (J) Representative images of the TUNEL assay in MLOY4 cells at indicated treatment, with quantitative data on the right (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.