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. 2024 May 19;57(10):e13657. doi: 10.1111/cpr.13657

FIGURE 5.

FIGURE 5

Preventing activating transcription factor 3 (ATF3) upregulation in aged mice alleviated ferroptosis‐induced cortical bone loss. (A) Western blot analysis of the levels of ATF3, transferrin receptor 1 (TFR1), glutathione peroxidase 4 (GPX4) and solute carrier family 7a member 11 (SLC7A11) at indicated time in the femur cortical bone of 3‐ and 19‐month‐old mice treated with solvent or JY‐2 (50 mg/kg/d, p.o.) for four weeks, with quantitative data on the right (n = 3). (B). Lipid peroxide colorimetric assay of femur cortical bone (n = 3). (C–E) Immunohistochemical staining for TFR1, SLC7A11 and GPX4 of the femur cortical bone (n = 3). (F) Representative Lillie staining (blue) of femur cortical bone n = 3. (G). Representative Perl's iron staining (blue) of femur cortical bone (n = 3). (H) Representative TUNEL staining of femur cortical bone (n = 3). (I) Representative images of micro‐CT reconstruction of femoral cortical bone, with quantitative data below (n = 5). M, months. *p < 0.05, **p < 0.01, ***p < 0.001.