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. 2024 Sep 4;15(29):2090–2099. doi: 10.1111/1759-7714.15309

FIGURE 4.

FIGURE 4

Effects of recombinant human AMH treatment on cell proliferation and cell cycle related gene expression in non‐small cell lung cancer cell lines overexpressing AMHR2. (a) Inhibitory effect of recombinant human AMH (rhAMH) on the proliferation of AMHR2‐overexpressing cell lines. Cell counts were obtained after 5 days of treatment with 20 or 50 ng/mL rhAMH. The y‐axis is normalized with control group mean values set to 100. Data from one representative of three independent experiments are shown, with error bars representing standard deviation. Statistical analysis was performed using Welch's t‐test. (*p < 0.05; **p < 0.01; ***p < 0.001). (b) Investigation of the effects of rhAMH on the genes using qPCR. The EGFP‐induced control A549 cell line (EGFP: left bar) as the control, AMHR2‐overexpressing A549 cell line (AMHR2: middle bar) and AMHR2‐overexpressing A549 cell line treated with 50 mg/mL rhAMH (AMHR2 + rhAMH: right bar) were compared. The qPCR was performed in triplicate, and data from one representative of six independent experiments are shown. The y‐axis is normalized with control group mean values set to 100, with error bars representing standard deviation. Statistical analysis was performed using Welch's t‐test. (*p < 0.05; **p < 0.01; ***p < 0.001).