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Multiple-step replication cycles in Vero and HEp-2 cells. Vero cells (top) or HEp-2 cells (bottom) were infected with rA2, rA2-Tr17, or rA2-C96G at an MOI of 0.1 PFU/cell. Aliquots of culture medium in duplicates were harvested at 24-h intervals, and the virus was quantitated by plaque assay on Vero cells.
