FIG. 5.

(A) Western blot of cell lysates infected with rA2, rA2-C96G, or rA2-Tr17. Total cellular proteins from infected cells were separated on 15% polyacrylamide gels containing 0.1% SDS, and the blots were hybridized separately with anti-M2-1 or anti-N antibodies. (B) Immunoprecipitation of labeled RSV polypeptides from infected HEp-2 or Vero cells. HEp-2 or Vero cells were infected with each virus as indicated, and the proteins were labeled with [³⁵S]methionine and [³⁵S]cysteine at 16 h postinfection. The labeled proteins were immunoprecipitated with anti-RSV or anti-M2-2 antibodies. The F1 protein from RSV-infected HEp-2 and Vero cells showed different gel mobilities, and less F1 was produced in rA2-Tr17-infected Vero cells in this particular experiment.