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. 2024 Oct 14;15:8849. doi: 10.1038/s41467-024-53072-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western Blot with 1E6 antibody combined with PNGase F treatment showing the presence of glycosylated HEV ORF2 protein (HEV ORF2g, 90 kDa) in supernatant of HEV-replicating Hep293TT cells (SN) and in the serum of Patient 1. No evidence of glycosylated protein in the kidney protein extract of Patient 1 (60 kDa bands), as observed in cell lysate of HEV-replicating Hep293TT cells containing intracellular non-glycosylated HEV ORF2intra (72 kDa bands). Detection of bands with higher molecular weight in kidney protein extract of Patient 1 likely corresponding to the immune complexes of HEV ORF2 proteins and immunoglobulins G (n = 2 blots). b Immunohistochemistry using 1E6 and P3H2 antibodies recognizing all the isoforms of HEV as well as P1H1, P2H1 and P2H2 mAbs recognizing only infectious HEV ORF2i. HEV-replicating Hep293TT cells, as well as liver (Patient 1 - liver) and kidney transplant tissue (Patient 1 - kidney). Scale bars: 50 µm (n = 3 experiments per tissue type, each condition in duplicate). Source data are provided as a Source Data file.