Figure 2.

mRNA editing in S. pyogenes SF370. (A) The A-to-I editing events identified in S. pyogenes SF370 (n = 27) were classified as synonymous or non-synonymous (i.e. recoding), and further sorted according to the effect on the amino acid level. (B) Left: mRNA editing consensus motifs in E. coli and K. pneumoniae. Middle: Schematic representation of the tRNA anticodon arms of the two TadA substrate tRNAs in S. pyogenes with the loop sequence shaded in grey and diverging nucleotides at position 35 and 37 highlighted in blue. Right: Consensus of the sequence context of the editing sites identified in S. pyogenes (± 5 nt) with sequence conservation shown in bits. (C) Secondary structure propensity around A-to-I editing target sites in S. pyogenes SF370 (blue) and control positions in coding sequences harbouring the YUAMGRA consensus sequence (orange, n = 2243).