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. 2024 Sep 12;52(18):11158–11176. doi: 10.1093/nar/gkae776

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — 5xFAD mice accumulate intron-containing pre-tRNAs. tRNAs were isolated from 7-month-old WT and 5xFAD mice and sequenced by Hydro-tRNA-seq. The percentage of normalized counts for precursor (A) and mature (B) tRNA is depicted, classified by amino acid and anticodon (pre-tRNA-Ala-TGC: t₆ = 2.692, P = 0.0360; pre-tRNA-Arg-TCT: t₅ = 3.364, P = 0.0200; pre-tRNA-Asp-GTC: t₆ = 2.635, P = 0.0388; pre-tRNA-Ile-TAT: t₅ = 2.986, P = 0.0306; pre-tRNA-Leu-CAA: t₆ = 3.440, P = 0.0138; pre-tRNA-Sec-TCA: t₆ = 2.496, P = 0.0468; pre-tRNA-Tyr-GTA: t₆ = 3.264, P = 0.0172) (tRNA-Asp-GTC: t₆ = 3.332, P = 0.0158; tRNA-Ile-AAT: t₆ = 3.814, P = 0.0088; tRNA-Ser-CGA: t₆ = 2.771, P = 0.0324). The red rectangles surrounding certain anticodons indicate that at least one of those pre-tRNA isodecoders has an intron. Data are means ± SEM. In all comparisons Student’s t-test was performed. Values were excluded when they were classified as outliers with either the ROUT or the Grubbs’ test from Graphpad Prism software. *P < 0.05 and **P < 0.01.