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. 2024 Sep 5;52(18):10754–10774. doi: 10.1093/nar/gkae741

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Crystal structure of double-stranded RNA with 2′-formamidouridine. (A) Sequence for crystal structure analysis. U^Br is 5-bromouridine and U^f is 2′-formamidouridine. This is a self-complementary sequence. (B–D) The result of X-ray structural analysis. The magenta-colored nucleoside was 2′-formamidourisine. (B) Overall structure of double-stranded RNA. (C) Base pairing of 2′-formamidouridine. (D) The sugar conformation of 2′-formamidouridine in double strand RNA.