Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 5;52(18):10754–10774. doi: 10.1093/nar/gkae741

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — RNAi on-target activity and off-target effects. (A) Vector design for evaluating on-target and off-target effects. (B) On-target activity and off-target effects. Upper RNA strand indicates the passenger strand, and the lower indicates the guide strand. Green graphs show on-target activity. The blue graphs show off-target effects. In the sequence, the red text indicates the position of the modifier. Relative luc activity (%) was calculated using the activity of Renilla luciferase and firefly luciferase transfected as a control. siRNA against green fluorescent protein (GFP) was used as control siRNA (siCont). The P-values were calculated using a Two-Way ANOVA test. Significance levels are indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001. Each experiment was repeated three times.