Table 1.

T _m value of the double strand RNAs with modification

Entry	Sequencea	T m (°C)b	ΔTm (°C)
1	5′-ACUGCUACGAU-3′ 3′-UGACGAUGCUA-5′	66.2 ± 0.1	-
2	5′-ACUGCUfACGAU-3′ 3′-UGACGAUGCUA-5′	58.6 ± 0.2	−7.6
3	5′-ACUGCUACGAU-3′ 3′-UGACGAfUGCUA-5′	59.7 ± 0.1	−6.5
4	5′-ACUGCUfACGAU-3′ 3′-UGACGAfUGCUA-5′	52.2 ± 0.2	−14.0
5	5′-GUCAUCGUAGC-3′ 3′-CAGUAGCAUCG-5′	67.6 ± 0.1	-
6	5′-GUCAUCfGUAGC-3′ 3′-CAGUAGCAUCG-5′	61.6 ± 0.1	−6.0
7	5′-GUCAUCGUAGC-3′ 3′-CAGUAGfCAUCG-5′	61.5 ± 0.1	−6.1
8	5′-GUCAUCfGUAGC-3′ 3′-CAGUAGfCAUCG-5′	55.2 ± 0.3	−12.4

^a X^f: 2′-formamidonucleoside,

^bThree measurements were taken, and the mean and standard error of the measurements were shown.