Table 2.

T _m value and of the double strand RNAs with mismatch base pair

Entry	Sequencea	T m (°C)b	ΔTm (°C)
1	5′-ACUGCUACGAU-3′ 3′-UGACGAUGCUA-5′	66.2 ± 0.1	-
9	5′-ACUGCUACGAU-3′ 3′-UGACGUUGCUA-5′	51.4 ± 0.1	−14.8
10	5′-ACUGCUACGAU-3′ 3′-UGACGGUGCUA-5′	61.7 ± 0.2	−4.5
11	5′-ACUGCUACGAU-3′ 3′-UGACGCUGCUA-5′	51.2 ± 0.1	−14.0
2	5′-ACUGCUfACGAU-3′ 3′-UGACGAUGCUA-5′	58.6 ± 0.2	-
12	5′-ACUGCUfACGAU-3′ 3′-UGACGUUGCUA-5′	44.4 ± 0.0	−14.2
13	5′-ACUGCUfACGAU-3′ 3′-UGACGGUGCUA-5′	56.7 ± 0.1	−1.9
14	5′-ACUGCUfACGAU-3′ 3′-UGACGCUGCUA-5′	44.5 ± 0.3	−14.1

^a U^f: 2′-formamidouridine.

^bThree measurements were taken, and the mean and standard error of the measurements were shown.