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. 2024 Sep 5;52(18):10754–10774. doi: 10.1093/nar/gkae741

Table 3.

Thermodynamic parameters of the double strand RNAs

Entry Sequencea ΔH°b (ΔΔH°) (kcal/mol) ΔS°b (ΔΔS°) (cal/mol·K) ΔG°37°Cb (ΔΔG°37°C) (kcal/mol)
1 5′-ACUGCUACGAU-3′ 3′-UGACGAUGCUA-5′ −118.8 ± 4.7 −350.9 ± 13.7 −10.0 ± 0.5
2 5′-ACUGCUfACGAU-3′ 3′-UGACGAUGCUA-5′ −104.1 ± 6.6 (+14.7) −314.5 ± 19.9 (+36.4) −6.6 ± 0.5 (+3.4)

a Uf: 2′-formamidouridine.

bThree measurements were taken, and the mean and standard error of the measurements were shown.