FIG. 8.
Sequence changes in the NRREpreC of the CH variant of HBV abolish activation of synthesis of viral RNA and DNA by PPARα-RXRα. (A) Autoradiogram of primer extension reactions used to quantify preC and pregenomic (preg) RNAs synthesized from the wild-type (WT) and CH variant HBV plasmids. Transient transfections of Huh7 cells and the concentrations of ligands for PPARα and RXRα added to the medium were as described for Fig. 5. (B) Autoradiogram of Southern blot analysis of cytoplasmic viral DNA present in the same cells. Numbers at the bottom are means ± standard errors of data quantified from three experiments similar to the one for which results are shown. RC DNA, relaxed circular DNA; DL DNA, duplex linear DNA; SS DNA, single-stranded DNA.