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. Author manuscript; available in PMC: 2024 Oct 14.
Published in final edited form as: Nat Chem Biol. 2022 Sep 22;18(11):1277–1286. doi: 10.1038/s41589-022-01138-9

Extended Data Fig. 9. Distal segments of the nascent peptide in the ribosomal exit tunnel contribute to EVN-dependent translation arrest within the emtAL ORF.

Extended Data Fig. 9.

a, The VFL motif within the EmtAL peptide affords only partial translation arrest. Top: gel electrophoretic analysis of accumulation of MMMAVF-tRNA and MMMAVFL-tRNA during in vitro translation of the truncated emtAL template at increasing concentrations of EVN. Bottom: quantification of the gel (two independent experiments) shoing the fraction of incomplete translation product (MMMAVF-tRNA). b, Toeprinting analysis of EVN-mediated ribosome stalling in templates encoding the E. faecium emtAL ORF where the identities of codons 2–4 were simultaneously modified by introducing single-nucleotide compensatory frame-shifting mutations (indicated by black arrows and red letters). Representative gels from two independent experiments are shown in panels a and b.