FIG. 2.

MHC binding affinity. TAP-deficient T2 cells were cultured for 16 h at 26°C to enhance expression of peptide-receptive cell surface molecules and then incubated with various concentrations of individual peptides at 37°C for 2 h, washed, and stained with fluorescein-conjugated anti-HLA-A2 antibody (One Lambda Inc.) and 1 μg of propidium iodide per ml. Data express the mean fluorescence intensity of live, propidium iodide-negative cells.