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In vitro cross-reactivity. Cryopreserved PBMC of five HCV-seronegative, healthy controls were stimulated for 3 weeks in vitro in the presence of IL-2, IL-7, IL-12, and 10 μg of IVNA-231, HPVL1-315, or WAG61 peptide per ml. Each CTL line was tested in a 6-h 51Cr release assay against C1R-A2 targets pulsed overnight with 10 μg of HCVNS3-1073 (▪), IVNA-231 (▨), HPVL1-315 (░⃞), or WAG161 (□) per ml. The 10% cutoff for a positive CTL assay is indicated by the horizontal line.
