FIG. 6.

(A) HCV_NS3-1073-specific CTLs recognize IV-infected target cells. IV PR8-infected C1R-A2 cells (filled circles) or uninfected C1R-A2 cells (open circles) were labeled with ⁵¹Cr for 1 h and used as target cells in a standard 6-h ⁵¹Cr release assay with the HCV_NS3-1073-specific CTL line derived from patient Chr-5. (B) IV_NA-231-specific CTL lines recognize IV-infected target cells. IV PR8-infected C1R-A2 cells (filled squares) or uninfected C1R-A2 cells (open squares) were labeled with ⁵¹Cr for 1 h and used as target cells in a standard 6-h ⁵¹Cr release assay with IV_NA-231-specific CTL effectors derived from healthy, HCV-negative blood donor HD-7. (C) Cytotoxic activity of an HCV_NS3-1073-specific CTL line from patient Chr-5 against peptide-pulsed or IV-infected target cells. The specific lysis at an effector/target ratio of 33:1 is shown.