TABLE 1.
Patient characteristics and HCVNS3-1073 and HCVNS3-1406 peptide-specific cytotoxic activity of T-cell lines derived from PBMC of patients with chronic hepatitis C
Patient | HCV genotype | HCV RNA (106 copies/ml) | ALTa (U/ml) | Liver
histologyb
|
Specific lysis
(%) ofc:
|
||
---|---|---|---|---|---|---|---|
Inflammatory score | Fibrosis score | HCVNS3-1073-loaded target cells | HCVNS3-1406-loaded target cells | ||||
Chr-1 | 1b | 30.9 | 25 | 7 | 0 | 48 | 29 |
Chr-2 | 2b | 6.1 | 35 | 9 | 0 | 59 | 53 |
Chr-3 | 2b | 4.7 | 14 | NDd | ND | 19 | 5 |
Chr-4 | 1b | 0.4 | 18 | 5 | 0 | 6 | 2 |
Chr-5 | 2b | 43 | 25 | ND | ND | 43 | 8 |
Chr-6 | 1a | 7.0 | 27 | 3 | 0 | 0 | 0 |
Chr-7 | UGe | 43.1 | 32 | ND | ND | 3 | 4 |
Chr-8 | 1b | 1.3 | 22 | ND | ND | 27 | 7 |
Chr-9 | 1b | 0.2 | 22 | ND | ND | 44 | 12 |
Chr-10 | 2b | 6.0 | 20 | ND | ND | 2 | 21 |
Chr-11 | 1a | 0.3 | 60 | 6 | 0 | 42 | 42 |
Chr-12 | 1a | 21.1 | 59 | 8 | 1 | 44 | 36 |
Chr-13 | 1a | 1.2 | 55 | 6 | 0 | 9 | 43 |
Chr-14 | 1a | 6.4 | 40 | 5 | 0 | 2 | 5 |
Chr-15 | 1b | 0.5 | 50 | 7 | 1 | 3 | 3 |
Chr-16 | 1a | 0.5 | 91 | 3 | 0 | 18 | 15 |
Chr-17 | 1a | 2.5 | 55 | 6 | 0 | 2 | 28 |
Chr-18 | 1a | 31.7 | 69 | ND | ND | 3 | 16 |
Chr-19 | 1a | 6.8 | 51 | 7 | 1 | 38 | 0 |
Chr-20 | 1a | 1.4 | 49 | 7 | 0 | 48 | 26 |
No. of patients positive/total (% positive) | 11/20 (55) | 11/20 (50) | |||||
Mean cytotoxicityf ± SD | 39 ± 12 | 29 ± 12 |
ALT, alanine aminotransferase.
Liver biopsy specimens were scored according the HAI score introduced by Knodell et al. (19b). Inflammatory score, i.e., histological inflammatory activity score, 0 to 14; fibrosis score, 0 to 4. All liver biopsies were performed within 3 months of the immunologic analysis.
The specific cytotoxic activity of peptide-stimulated cell lines was tested at an effector/target cell ratio of 30:1. Responses with a specific lysis of >10% were considered to be positive and are shown in boldface. Cell lines tested for HCVNS3-1073-specific cytotoxicity had been stimulated with peptide HCVNS3-1073; cell lines tested for HCVNS3-1406-specific cytotoxicity had been stimulated with peptide HCVNS3-1406.
ND, not done.
UG, unable to genotype.
The mean cytotoxicity of all positive T-cell lines was calculated.