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. 1986 Oct 1;239(1):147–153. doi: 10.1042/bj2390147

Electron microscopy of cervical, gastric and bronchial mucus glycoproteins.

J K Sheehan, K Oates, I Carlstedt
PMCID: PMC1147252  PMID: 3800974

Abstract

Mucus glycoproteins (mucins) were obtained from human cervical and pig gastric mucus as well as from chronic-bronchitic sputum after low-shear extraction. The mucus gel was solubilized in guanidinium chloride supplemented with proteinase inhibitors, and the macromolecules were purified by using isopycnic density-gradient centrifugation. The macromolecules were spread in monolayers of benzyldimethylalkyl-ammonium chloride and studied with electron microscopy after staining with uranyl acetate and/or shadowing with platinum/carbon. The mucins appeared as flexible linear threads with lengths varying from approx. 200 nm to about 400 nm. No regularly branched or star-shaped structures were observed. The macromolecular architecture of cervical, respiratory and gastric mucins is thus similar.

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Selected References

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  1. Allen A., Hutton D. A., Mantle D., Pain R. H. Structure and gel formation in pig gastric mucus. Biochem Soc Trans. 1984 Aug;12(4):612–615. doi: 10.1042/bst0120612. [DOI] [PubMed] [Google Scholar]
  2. Allen A., Hutton D. A., Pearson J. P., Sellers L. A. Mucus glycoprotein structure, gel formation and gastrointestinal mucus function. Ciba Found Symp. 1984;109:137–156. doi: 10.1002/9780470720905.ch10. [DOI] [PubMed] [Google Scholar]
  3. Carlstedt I., Lindgren H., Sheehan J. K., Ulmsten U., Wingerup L. Isolation and characterization of human cervical-mucus glycoproteins. Biochem J. 1983 Apr 1;211(1):13–22. doi: 10.1042/bj2110013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlstedt I., Sheehan J. K. Is the macromolecular architecture of cervical, respiratory and gastric mucins the same? Biochem Soc Trans. 1984 Aug;12(4):615–617. doi: 10.1042/bst0120615. [DOI] [PubMed] [Google Scholar]
  5. Carlstedt I., Sheehan J. K. Macromolecular properties and polymeric structure of mucus glycoproteins. Ciba Found Symp. 1984;109:157–172. doi: 10.1002/9780470720905.ch11. [DOI] [PubMed] [Google Scholar]
  6. Creeth J. M., Cooper B. Studies on the molecular weight distributions of two mucins. Biochem Soc Trans. 1984 Aug;12(4):618–621. doi: 10.1042/bst0120618. [DOI] [PubMed] [Google Scholar]
  7. Fisher H. W., Williams R. C. Electron microscopic visualization of nucleic acids and of their complexes with proteins. Annu Rev Biochem. 1979;48:649–679. doi: 10.1146/annurev.bi.48.070179.003245. [DOI] [PubMed] [Google Scholar]
  8. GIBBONS R. A., GLOVER F. A. The physicochemical properties of two mucoids from bovine cervical mucin. Biochem J. 1959 Oct;73:217–225. doi: 10.1042/bj0730217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harding S. E., Rowe A. J., Creeth J. M. Further evidence for a flexible and highly expanded spheroidal model for mucus glycoproteins in solution. Biochem J. 1983 Mar 1;209(3):893–896. doi: 10.1042/bj2090893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jenssen A. O., Harbitz O., Smidsrød O. Electron microscopy of mucin from sputum in chronic obstructive bronchitis. Eur J Respir Dis. 1980 Apr;61(2):71–76. [PubMed] [Google Scholar]
  11. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  12. Mikkelsen A., Stokke B. T., Christensen B. E., Elgsaeter A. Flexibility and length of human bronchial mucin studied using low-shear viscometry, birefringence relaxation analysis, and electron microscopy. Biopolymers. 1985 Sep;24(9):1683–1704. doi: 10.1002/bip.360240904. [DOI] [PubMed] [Google Scholar]
  13. Rose M. C., Voter W. A., Brown C. F., Kaufman B. Structural features of human tracheobronchial mucus glycoprotein. Biochem J. 1984 Sep 1;222(2):371–377. doi: 10.1042/bj2220371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sheehan J. K., Carlstedt I. Hydrodynamic properties of human cervical-mucus glycoproteins in 6M-guanidinium chloride. Biochem J. 1984 Jan 1;217(1):93–101. doi: 10.1042/bj2170093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sheehan J. K., Carlstedt I. The effect of guanidinium chloride on the behaviour of human cervical-mucus glycoproteins. Evidence for unfolding regions of ordered structure in 6M-guanidinium chloride. Biochem J. 1984 Jul 15;221(2):499–504. doi: 10.1042/bj2210499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slayter H. S., Lamblin G., Le Treut A., Galabert C., Houdret N., Degand P., Roussel P. Complex structure of human bronchial mucus glycoprotein. Eur J Biochem. 1984 Jul 16;142(2):209–218. doi: 10.1111/j.1432-1033.1984.tb08273.x. [DOI] [PubMed] [Google Scholar]

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