Figure 8.

Representative photomicrographs and characterization of successfully growing PDOs

(A–C) Bright-field images of live culture PDOs derived from cervical adenocarcinoma (A) and cervical squamous cell carcinoma (B and C).

(D and H) Hematoxylin and eosin (HE) staining of formalin-fixed, paraffin-embedded PDOs derived from cervical adenocarcinoma (D) and cervical squamous cell carcinoma (H).

(E) Periodic acid-Schiff (PAS) staining of PDOs derived from cervical adenocarcinoma.

(F and G) Immunofluorescent staining of PDOs derived from cervical adenocarcinoma for PAX8 and Ki67.

(I and J) Immunofluorescent staining of PDOs derived from cervical squamous cell carcinoma for P63 and Ki67. Scale bar = 100 μm.