REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Advanced DMEM/F12 medium	Gibco	12634010
GlutaMAX	Gibco	35050061
Forskolin	Bio-Techne	1099
FGF7	PeproTech	100-19
FGF10	PeproTech	100-26
B27 supplement	Gibco	17504-044
Nicotinamide	Sigma	N0636
N-acetylcysteine	Sigma	A9165
ROCK inhibitor	AbMole BioScience	Y27632
EGF	PeproTech	AF-100-15
A83-01	Tocris Bioscience	2939
p38 inhibitor SB202190	Sigma	7067
HEPES	Gibco	15630080
TrypLE	Gibco	12605-010
Cultrex reduced growth factor basement membrane extract, type 2	Trevigen	3533-005-02
Primocin	InvivoGen	Ant-pm-1
P/S	Corning	30-002-ci
FBS	Corning	35-010-cv
RPMI 1640	Corning	10-040-cv
Collagenase	Sigma	C9407
Hyaluronidase	Sigma	H6254
Trypsin-EDTA	Corning	25200-05-30
Dulbecco’s PBS	Corning	21-030-cv
BSA	Corning	15260–037
Lymphoprep	STEMCELL Technologies	7851
Zeocin	InvivoGen	ant-zn
DNase I	Sigma	D4513-1vl
Puromycin	Sigma	P8833-10mg
PluriSTEM Dispase-II solution	Sigma	SCM133
Experimental models: Cell lines
Noggin-expressing 293T cell line	Baylor College of Medicine Digestive Diseases Center	N/A
R-spondin1-expressing 293T cell line	Applied Biological Materials	T3156
Other
Low-protein-binding microcentrifuge tubes	Thermo Fisher Scientific	90410
Low-retention pipette tip, 1000 μL	Alkali Scientific	ft1250
Low-retention pipette tip, 200 μL	Genesee Scientific	23-412
Eppendorf Xplorer plus	Eppendorf	4861000724
Sterile cell strainer, 100 μm	Corning	352360
Sterile cell strainer, 40 μm	Thermo Fisher Scientific	22363547
Multiwell culture plates, 24-well	Sarstedt	83.3922
Multiwell culture plates, 12-well	Sarstedt	83.3921
Petri dish, 100 × 15 mm	Sarstedt	83.3906
Petri dish, 60 × 15 mm	Sigma	cls430166
Conical tubes, 50 mL	Thermo Fisher Scientific	14-955-240
Conical tubes, 15 mL	MIDSCI	#c15b
Serological pipettes, 10 mL	Thermo Fisher Scientific	13–678-11d
Serological pipettes, 25 mL	Alkali Scientific	SP225-B
SB-12L shaking water baths	Benchmark Scientific	SB0012
Cytobrush samplers	CooperSurgical	c0012
Nonfiltered pipette tips, 200 μL	MIDSCI	PR-200RFL
Hemocytometer	Bright-Line	Z359629
TC20 automated cell counter	Bio-Rad	1450102
Cytation 5 cell imaging multimode reader	BioTek	N/A