Part B: growth medium with supplements

Reagent	Final concentration	Stock	Amount added
Growth medium stock	–	–	8.8 mL
R-spondin1–conditioned medium	10%	–	1 mL
Noggin-conditioned medium	2%	–	200 μL
FGF10	100 ng/mL	100 μg/mL	10 μL
FGF7	25 ng/mL	250 μg/mL	1 μL
EGF	50 ng/mL	50 μg/mL	10 μL
HGF	50 ng/mL	10 μg/mL	5 μL
Heregulin beta-1	40 ng/mL	40 μg/mL	10 μL
CHIR	300 nM	3 mM	1 μL
Hydrocortisone	500 ng/mL	500 μg/mL	10 μL

Store at 4°C for no more than 1 month. Do not freeze. Make fresh.