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. 2024 Sep 20;27(10):111008. doi: 10.1016/j.isci.2024.111008

Figure 1.

Figure 1

OptoDArG-evoked TRPC6 current kinetics strongly depend on the intensity of the light source

Electrophysiological whole-cell measurements of TRPC6 overexpressing HEK293T cells in the presence of 30 μM OptoDArG.

(A) Summaries of current densities (“Curr. dens.”) at potentials of ±100 mV evoked by light using a xenon lamp (red) or a LED (black). First small boxplots represent current densities in the presence of blue light (445 nm) which establishes trans-OptoDArG and second boxplots represent maximal current densities in the presence of UV light (365 nm) which establishes cis-OptoDArG. No significant differences between trans or cis-OptoDArG-induced current densities were observed using xenon or LED light source, respectively (Mann-Whitney U test).

(B and C) Representative current density (“Curr. dens.”)-time courses ±100 mV and current density-voltage relations using xenon lamp (B) or LED (C) for photoswitching. The arrows indicate the time point at which the trans- and the maximal cis-OptoDArG-induced current density-voltage relations were selected. Bars over current density-time courses indicate the application of light of the wavelength 445 nm (blue) and of 365 nm (magenta).

(D) Representative normalized time courses of TRPC6 inward currents at constant holding potential of −60 mV during photoswitching from blue light (blue bar) to UV light (magenta bar) and back to blue light (blue bar) using a xenon lamp (red) or LED (black).

(E and F) Summaries of half-life time constants (τH) of the activation (E) and deactivation (F) kinetics (∗∗∗p < 0.001; Mann-Whitney U test).

(G) Representative normalized time courses of TRPC6 inward currents at constant holding potential of −60 mV induced by photoswitching from blue light to UV light using xenon lamp (red) or LED (black). The duration of light application (blue and magenta bar) is indicated.

(H and I) Summaries of half-life time constants (τH) of the fast (H) and slow (I) inactivation kinetics. (∗p < 0.05, ∗∗∗p < 0.001; Mann-Whitney U test). (E, F, H, and I) Data are displayed as boxplots and interquartile ranges. Numbers over boxplots indicate number of measured cells.