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. 2024 Sep 20;27(10):111008. doi: 10.1016/j.isci.2024.111008

Figure 3.

Figure 3

TRPC6 current densities elicited by photoswitchable and non-photoswitchable TRPC6 channel activators

Electrophysiological whole-cell measurements of TRPC6 overexpressing HEK293T cells.

(A) Summaries of current densities (“Curr. dens.”) at potentials of ±100 mV evoked by application 100 μM OAG, of 10 μM GSK 1702934A (“GSK”), of blue light and UV light using LEDs in the presence of OptoDArG, PhoDAG or OptoBI-1. First small boxplots represent current densities before application of OAG or GSK or induced by trans-OptoDArG, trans-PhoDAG or trans-OptoBI-1 and second boxplots maximal current densities induced by OAG, cis-OptoDArG, cis-PhoDAG, GSK or cis-OptoBI-1. Significant differences were calculated using Kruskal-Wallis test. Brown asterisks indicates significant differences compared to the OAG-induced current densities and green asterisks compared to OptoDArG-induced current densities (∗∗p < 0.01, ∗p < 0.05). Data are displayed as boxplots and interquartile ranges. Numbers over boxplots indicate number of measured cells.

(B–F) Representative current density (“Curr. dens.”)-time courses at ±100 mV and current density-voltage relations. Bars indicate bath application of OAG (B, brown bar) or GSK (E, violet bar) or of blue light (C, D, F; blue bar) or UV light (C, D, F; magenta bar) using LEDs. The arrows indicate the time point at which the current density-voltage relations were selected.