Fig. 7.

Type of exercise and genotype impact the expression of MyHC isoforms IIa, IIx and IIb in male mice. 12-week-old WT, Fn14^−/−, SOD1^G93A and SOD1^G93A;Fn14^−/− males were either placed on the rotarod (A-D) or performed the grid test (E–H) daily for 5 consecutive days. Tibialis anterior (TA) muscles were harvested approximately 2 h after the last bout of exercise. A-D qPCR analysis of MyHC IIa, IIx and IIb mRNA expression in unexercised and rotarod-exercised WT (A), SOD1^G93A (B), Fn14^−/− (C) and SOD1^G93A;Fn14^−/− (D) males. Data are scatter dot plot mean ± SEM, n = 3–4 animals per experimental group, two-way ANOVA, ns = not significant, ***p < 0.001. E–H qPCR analysis of MyHC IIa, IIx and IIb mRNA expression in unexercised and grid test-exercised WT (E), SOD1^G93A (F), Fn14^−/− (G) and SOD1^G93A;Fn14.^−/− (H) males. Data are scatter dot plot mean ± SEM, n = 3–5 animals per experimental group, two-way ANOVA, ns = not significant, *p < 0.05