Fig. 8.
Type of exercise and genotype impact the expression of MyHC isoforms IIa, IIx and IIb in female mice. 12-week-old WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− females were either placed on the rotarod (A-D) or performed the grid test (E–H) daily for 5 consecutive days. Tibialis anterior (TA) muscles were harvested approximately 2 h after the last bout of exercise. A-D qPCR analysis of MyHC IIa, IIx and IIb mRNA expression in unexercised and rotarod-exercised WT (A), SOD1G93A (B), Fn14−/− (C) and SOD1G93A;Fn14−/− (D) females. Data are scatter dot plot mean ± SEM, n = 4–8 animals per experimental group, two-way ANOVA, ns = not significant, ***p < 0.001. ***p < 0.0001. E–H qPCR analysis of MyHC IIa, IIx and IIb mRNA expression in unexercised and grid test-exercised WT (E), SOD1G93A (F), Fn14−/− (G) and SOD1G93A;Fn14.−/− (H) females. Data are scatter dot plot mean ± SEM, n = 3–8 animals per experimental group, two-way ANOVA, ns = not significant, ***p < 0.001. ***p < 0.0001