Fig. 9.
Type of exercise, genotype and sex impact muscle fibre size. 12-week-old WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− males and females either performed the grid test or were placed on the rotarod daily for 5 consecutives days. Gastrocnemius muscles were harvested approximately 2 h after the last bout of exercise. A Quantification of myofiber area of laminin-stained cross-sections of gastrocnemius muscles from 12-week-old unexercised and rotarod-exercised WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− males. Data are dot plot and mean, n = 3–4 animals per experimental group (> 1100 myofibers per experimental group), two-way ANOVA, ns = not significant, ****p < 0.0001. B Quantification of myofiber area of laminin-stained cross-sections of gastrocnemius muscles from 12-week-old unexercised and grid test-exercised WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− males. Data are dot plot and mean, n = 3–4 animals per experimental group (> 1100 myofibers per experimental group), two-way ANOVA, *p < 0.05, ****p < 0.0001. C Quantification of myofiber area of laminin-stained cross-sections of gastrocnemius muscles from 12-week-old unexercised and rotarod-exercised WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− females. Data are dot plot and mean, n = 3–4 animals per experimental group (> 1400 myofibers per experimental group), two-way ANOVA, ns = not significant, ****p < 0.0001. D Quantification of myofiber area of laminin-stained cross-sections of gastrocnemius muscles from 12-week-old unexercised and grid test-exercised WT, Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/− females. Data are dot plot and mean, n = 3–4 animals per experimental group (> 1100 myofibers per experimental group), two-way ANOVA, **p < 0.01, ****p < 0.0001