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. 2001 Dec;75(23):11408–11416. doi: 10.1128/JVI.75.23.11408-11416.2001

FIG. 1.

FIG. 1

Molecular characterization of the nuclear pool of NF-κB in promonocytic cells and analysis of HIV replication. (A) Immunoblotting of cytosolic (C) and nuclear (N) extracts in SFFV- and IκBα-2N-expressing U937 cells with anti-RelA, anti-RelB, and anti-p100/p52 antibodies. (B) HIV p24 values in the culture supernatants of HIV LAV-BN-infected SFFV- and IκBα-2N-expressing U937 cells at day 30 postinfection. (C) Immunoblotting of cytosolic (C) and nuclear (N) extracts from THP1 cells with anti-RelA, anti-RelB, and anti-p100 antibodies. (D) HIV p24 values in the culture supernatants of THP1 cells infected with HIV HXB2D-wt and HXB2D-ΔκB at day 14 postinfection.