FIG. 5.

NF-κB, but not NFAT, present in the nuclei of human macrophages binds the NF-κB cis-acting motifs of the HIV LTR. (A) NF-κB DNA binding. Three micrograms of nuclear extracts either from peripheral blood lymphocytes (PBLs) treated for 30 min either with TNF (10 ng/ml) for NF-κB binding or with PMA (20 ng/ml) plus ionomycin (3.5 μg/ml) for NFAT binding or from 5-day-old plastic-adhered human macrophages was incubated with ³²P-labeled DNA probes as indicated. Competition of DNA binding to the labeled oligonucleotide was analyzed in the presence of a 20-fold excess of nonlabeled DNA probe as indicated. (B) NFAT DNA binding. Extracts and experimental conditions were the same as for the experiment for which results are shown in panel A.