Skip to main content
. 2001 Dec;75(23):11408–11416. doi: 10.1128/JVI.75.23.11408-11416.2001

FIG. 6.

FIG. 6

The HIV-1AD ΔκB provirus does not undergo transcription in MDM. (A) (Upper panel) HIV DNA PCR amplification from uninfected (NI) and HIV-infected MDM at different times after infection. The 318-bp fragment encompassing the LTR-Gag sequence is indicated (LTR-Gag). (Lower panel) Human β-actin PCR amplification within the same samples. (B) HIV DNA integration in MDM that were infected with HIV-1AD wt or ΔκB. The viral integrated DNA LTR fragment is indicated (LTR). Genomic DNA from ACH2 cells was used as a positive control (lane 2). NI, not infected. (C) (Upper panel) PCR amplification of early HIV transcripts (tat/rev, env) in MDM that were infected with HIV-1AD wt or ΔκB. The amplified 219-bp fragment was visualized by Southern blot hybridization with a γ-32P-labeled U5 LTR internal primer. cDNA from ACH2 was used as a positive control. (Lower panel) Human β-actin hybridization in the same samples.