FIG. 1.

Vaccinia virus strain Copenhagen protects cells from anti-Fas-mediated death. Jurkat cells were either mock infected or infected with either vaccinia virus strain Copenhagen or vaccinia virus strain Western Reserve at an MOI of 10. Following 5 h of infection, cells were treated with 250 ng of anti-Fas antibody/ml to induce apoptosis, and cell death was monitored 8 h later by ⁵¹Cr release. As controls, Jurkat cells that overexpress SPI-2 and Bcl-2 were also treated with anti-Fas, and Jurkat cells were pretreated for 30 min with 100 μM zVAD.fmk prior to addition of anti-Fas. Standard deviations were generated from three replicates.