FIG. 4.

In vitro cytotoxic assays of HBcAg-specific CD4⁺ Th1 T-cell clones 124 and 135. JAM assays were performed as described in Materials and Methods. Peptide-loaded autologous EBV-transformed LCLs were used as target cells. Assays were set up at different effector/target ratios (90/1, 30/1, 10/1, 3.3/1, 1/1, and 0/1). All conditions were tested in triplicate. Specific lysis of target cells at different effector/target ratios is shown. Spontaneous lysis was always less than 20%.