Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Nov 1;239(3):517–522. doi: 10.1042/bj2390517

Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus.

P Giardina, M G de Biasi, M de Rosa, A Gambacorta, V Buonocore
PMCID: PMC1147317  PMID: 3827812

Abstract

Glucose dehydrogenase has been purified to homogeneity from cell extracts of the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme utilizes both NAD+ and NADP+ as coenzyme and catalyses the oxidation of several monosaccharides to the corresponding glyconic acid. Substrate specificity and oxidation rate depend on the coenzyme present; when NAD+ is used, the enzyme binds and oxidizes specifically sugars presenting equatorial orientation of hydroxy groups at C-2, C-3 and C-4. The Mr of the native enzyme is 124,000 and decreases to about 60,000 in the presence of 6 M-guanidinium chloride and to about 30,000 in the presence of 5% (w/v) SDS. The enzyme shows maximal activity at pH 9, 77 degrees C and 20 mM-Mg2+, -Mn2+ or -Ca2+ and is fairly stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol or acetone.

Full text

PDF
521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avigad G., Alroy Y., Englard S. Purification and properties of a nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogeanse from Gluconobacter cerinus. J Biol Chem. 1968 Apr 25;243(8):1936–1941. [PubMed] [Google Scholar]
  2. BACH J. A., SADOFF H. L. Aerobic sporulating bacteria. I. Glucose dehydrogenase of Bacillus cereus. J Bacteriol. 1962 Apr;83:699–707. doi: 10.1128/jb.83.4.699-707.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campbell D. P., Carper W. R., Thompson R. E. Bovine liver glucose dehydrogenase: isolation and characterization. Arch Biochem Biophys. 1982 Apr 15;215(1):289–301. doi: 10.1016/0003-9861(82)90307-1. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. De Rosa M., Gambacorta A., Nicolaus B., Giardina P., Poerio E., Buonocore V. Glucose metabolism in the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. Biochem J. 1984 Dec 1;224(2):407–414. doi: 10.1042/bj2240407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fujita Y., Ramaley R., Freese E. Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis. J Bacteriol. 1977 Oct;132(1):282–293. doi: 10.1128/jb.132.1.282-293.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hochstein L. I. The metabolism of carbohydrates by extremely halophilic bacteria: glucose metabolism via a modified Entner-Doudoroff pathway. Can J Microbiol. 1974 Aug;20(8):1085–1091. doi: 10.1139/m74-170. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Pauly H. E., Pfleiderer G. D-glucose dehydrogenase from Bacillus megaterium M 1286: purification, properties and structure. Hoppe Seylers Z Physiol Chem. 1975 Oct;356(10):1613–1623. doi: 10.1515/bchm2.1975.356.2.1613. [DOI] [PubMed] [Google Scholar]
  10. Price C. P., Spencer K. A rapid kinetic assay for glucose using glucose dehydrogenase. Ann Clin Biochem. 1979 Mar;16(2):100–105. doi: 10.1177/000456327901600120. [DOI] [PubMed] [Google Scholar]
  11. Pulich W. M., Baalen C. Purification and characterization of glucose dehydrogenase from a heterotrophically grown blue-green alga. Plant Physiol. 1976 Sep;58(3):393–397. doi: 10.1104/pp.58.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sundaram P. V., Blumenberg B., Hinsch W. Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor. Clin Chem. 1979 Aug;25(8):1436–1439. [PubMed] [Google Scholar]
  13. Vasantha N., Uratani B., Ramaley R. F., Freese E. Isolation of a developmental gene of Bacillus subtilis and its expression in Escherichia coli. Proc Natl Acad Sci U S A. 1983 Feb;80(3):785–789. doi: 10.1073/pnas.80.3.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. de Rosa M., Gambacorta A., Bu'lock J. D. Extremely thermophilic acidophilic bacteria convergent with Sulfolobus acidocaldarius. J Gen Microbiol. 1975 Jan;86(1):156–164. doi: 10.1099/00221287-86-1-156. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES