FIG. 5.
DNA-induced anti-E antibody and protective immunity in gene knockout mice. Gene knockout mice and wild-type (WT) controls (C57BL/6) were given intramuscular (i.m.) or gene gun injections of pE three times at 3-week intervals. Two weeks after the last immunization, mice were challenged with 50 LD50 of JEV Beijing-1. The concentration of anti-E antibodies was determined as described in the Fig. 2 legend. The percentage of animals in each group that survived JEV challenge is indicated above each column.