Figure 3.

CD44 expression in NZB/W F1 mice with progressive LN. Left panel: Representative images showing CD44 expression in NZB/W F1 mice with progressive disease, where 8, 16, 25 and 32 weeks of age represent different stages of disease, namely pre-nephritis, emergence of anti-dsDNA antibodies, active nephritis and severe disease respectively (n = 6 mice per timepoint). Original magnification x200. Right panel: scatterplot showing CD44-positive staining as a percentage of the whole image area using ImageJ software. Each dot represents an individual mouse. Horizontal line represents the mean for each group. ***P<0.001, compared to 8 weeks of age, ^## P<0.001, 16 weeks vs 32 weeks. Data analysed using Kruskal-Wallis test followed by Dunn’s multiple comparison post-test.