FIG. 1.

Schematic representation of the RV gene order in the negative-strand genomic RNA. (A) Recombinant RV SAD-L16, possessing the authentic sequence of SAD-B19 (6), which has an arginine (R333) at position 333 of the G protein. (B) Recombinant RV SAD-D29, possessing an aspartic acid (D333) at position 333 of the G protein. Amino acid sequences around the LC8 dynein light chain-binding site of the P protein (positions 138 to 150) are presented. The P protein sequence KSTQT (positions 144 to 148) is identical to the K/RXTQT motif found in a variety of proteins interacting with LC8. The ΔP7 and ΔP11 mutants constructed in the backbone of SAD-L16 and SAD-D29 contain the indicated deletions of 7 or 11 amino acids at the LC8 binding site of the respective P proteins.