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Pathogenicity of recombinant RVs. One-week-old suckling mice were inoculated with recombinant RVs SAD-L16 (L16), L-ΔP7 (L7), L-ΔP11 (L11), SAD-D29 (D29), D-ΔP7 (D7), and D-ΔP11 (D11) at a dose of 100 or 100,000 FFU/mouse i.m. Mice were observed daily for 21 days, and results recorded at the end of the experiment are presented. No rabies symptoms were observed in any group of mice inoculated with recombinant RVs possessing an aspartic acid at position 333 of the G protein.
