Fig. 4. pSRS shifts the dendritic cell signature towards a pro-inflammatory phenotype and promotes the accumulation of the TD1 GZMB high effector subset.

A Frequency of CD8 T cells of total live CD45+ cells across all patient tumors, as measured by flow cytometry. Medians are shown, and statistical comparisons were performed using two-sided unpaired Mann Whitney U test (n= 34). B Single cell RNAseq UMAP projection of sub-clustered CD8 T cells. C Normalized gene expression of selected differentially expressed genes defining each CD8 T cell cluster. D, E Relative cluster distribution D and total frequency of CD45+ live cells E of CD8 T cells subsets in Res (n= 18) and pSRS (n= 13) patient tumors. Medians are shown for each summary plot and statistical comparisons were performed using Mann Whitney U test between Res and pSRS groups. F, G Metascape pathway enrichment analysis in CD8 T cell subsets from pSRS tumors. Top five significant pathways are shown for F Stem-like CD8 T cells and G TD1 GZMB high CD8 T cells. H Transcriptional comparison of CD8 T cell subsets across Res and pSRS groups. The color and size of the circles represent the normalized expression and proportion of cells expressing that gene, respectively. I, J NicheNet sender-receiver analysis showing the top 3-4 predicted ligand-receptor interactions between activated DCs and CD8 T cell subsets in I Res or J pSRS. The thickness of circles and connections represents the prioritization score and interaction strength for each L-R pair. P values are shown. Source data are provided as a Source Data file and are available on the NCBI Gene Expression Omnibus (GEO) database.