Figure 8.

RNAi-mediated gene knockdown of CSDIR.A, percentage relative expression of CSDIR gene after 24 h post-injection, confirming CSDIR gene knockdown, GFP was used as a control (n = 10). Each bar represents an individual mosquito sample (n = 7). B, percentage survival curve in control (n = 14) and test (n = 16) group. C, a bar graph showing %sex ratio in the control (male(n = 66), female (n = 57)) and test (male (n = 48), female (n = 50)) group depicting CSDIR gene knockdown has not affected mosquito fitness. D, a bar graph showing a significant reduction in enzymatic activity against α-naphthyl acetate and β-naphthyl acetate in CSDIR knockdown mosquitoes compared to GFP knockdown mosquitoes. The points above the bar show the standard deviation. Statistical difference was calculated using an unpaired t test. E, a bar graph showing % mortality after 24 h post-exposure of deltamethrin and malathion in CSDIR and GFP knockdown mosquitoes. The data shows the mean of two biological replicates. Statistical difference was calculated using an unpaired t test. F, a graph showing an increase in K_m value against α-naphthyl acetate and β-naphthyl acetate in CSDIR knockdown mosquitoes compared with GFP knockdown mosquitoes.