Fig. 2.

Protein turnover of hDANs.A, SILAC label incorporation across all time points calculated as H/(L + H). Only proteins with ratio in two out of three replicates were considered. Number of proteins depicted are n = 5806, 5995, 6072, 6165, 6302, 6036 for their corresponding time points. B, distribution of total proteins with measured half-lives (4397) organized in eight different cellular compartments according to their GOCC annotation. Number of protein groups assigned to each compartment is given in brackets. Significant ∗p ≤ 0.05, ∗∗p ≤ 0.01, and ∗∗∗p ≤ 0.001, t test. For the boxplots, center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. C, range of log₂ scale half-lives of hDANs proteins, with a median of 97 h. D, schematic illustration of the TOM complex (upper left panel), the 20S proteasome (upper right panel), and the respiratory chain (lower panel) showing half-lives for individual components of each complex. Proteins are color coded as a gradient from light pink (shortest half-life) to dark blue-black (longest half-life).