Fig. 4.

Quantitative proteome analysis of hDANs cultured in microfluidic devices. A, schematic illustration of the experimental set up (right label) for time course experiment. Boxplots depicting SILAC label incorporation (left panel) across all time points in the soma well and the axon well calculated as H/(L +M+ H) and M/(L +M+ H). Only those proteins with label incorporation in two out of three replicates were considered. Color coding: blue for label in the soma side and orange for label in the axon side, with increasing darkness for longer time points. B, schematic illustration of the experimental set up (right label) for protein synthesis inhibition by CHX experiment. Boxplots depicting label incorporation (left panel) across all samples in the soma side and the axon side calculated as H/(L +M+ H) and M/(L +M+ H). Only those proteins with label incorporation in two out of three replicates were considered. Color coding: blue tone for soma side label, orange tone for the axon side label. For the boxplots, center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots.