Fig. 2.

Good biocompatibility of Ber-lipo in vitro and in vivo. (A, B) CCK-8 assays in RAW264.7 and HUVEC cells, respectively (n = 3). (C) Live/dead cell staining in HUVEC. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells. Images were taken at 100 × magnification. Scale bar, 250 μm. (D) Cell skeleton staining in HUVEC. Images were captured using 40 × oil lens of confocal microscope (LSM710, Zeiss, Germany). Green, FITC phalloidine. Blue, DAPI. Scale bar, 50 μm. (E) Hemolysis ratio assays of Ber-lipo (n = 3). (F) Histopathological evaluation of heart, liver, spleen, and kidney of LPS-induced ALI mice using HE staining.