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. 2024 Oct 1;43:406–422. doi: 10.1016/j.bioactmat.2024.09.020

Fig. 5.

Fig. 5

Fig. 5

Ber-lipo relieved LPS-induced ALI in vivo. (A) Schematic illustrating of animal experimental design. (B) Histopathology of lung tissues by H&E staining. Scale bar, 50 μm. Black arrows, inflammatory cell infiltration. Red arrows, disruption of alveolar structure. green arrows, bloody exudation. (C) Pathological scores of HE staining results (n = 3). (D) Changes of murine body weight loss among all groups for 3 d (n = 3). (E) Lung index of ALI mice (n = 3). (F, G) Total protein content and cell number in BALF (n = 3). (H–J) Pulmonary function of mice (n = 3). (K–N) The normalized mRNA expression of tight junction factors, ZO-1, VE-Cadherin, Claudin 5, and E-Cadherin, by qRT-PCR (n = 3). Internal control, α-Tubulin. (O) The Protein expression of tight junction factors, ZO-1, VE-Cadherin, Claudin 5, and E-Cadherin, by Western blot. (P) The normalized protein expressions calculated by using ImageJ software (n = 3). Internal control, β-actin. (Q) The distribution of tight junction proteins, ZO-1, endothelium-specific markers (VE-Cadherin and Claudin 5), and epithelium-specific marker (E-Cadherin), by immunofluorescent staining in lung tissues. Images were magnified at 100 × . Scale bar, 100 μm ∗P < 0.05 vs Control. #P < 0.05 vs Model. †P < 0.05.