Fig. 6.

Ber-lipo maintained balance between M1 and M2 macrophage polarization in lungs of ALI mice. (A) The expression patterns and distributions of M1 (CD86 and iNOS) and M2 (CD163 and CD206) markers by immunofluorescent staining. Images were taken under 40 × oil immersion lens of confocal macroscope. Scale bar, 50 μm. (B) Protein bands of CD86 (M1) and CD163 (M2) by Western blot. (C, D) Statistical analysis of proteins calculated by imageJ (n = 3). Internal control, β-actin. (E–M) The normalized transcriptional expressions of M1-associated genes (CD86, iNOS, TNF-α, IL-6, and IL-1β) and M2-associated ones (CD163, CD206, CCL22, and CCL24) by qRT-PCR (n = 3). Internal control, α-Tubulin. ∗P < 0.05 vs Control. #P < 0.05 vs Model. †P < 0.05.